The Role of Nuclear Components in Dengue Virus Replication
Their Interaction with the Core Protein
Keywords:
Dengue virus, Core protein, Nucleolin, Nucleophosmin, Histone H2B, RNA helicase DDX5, Viral replication, Nuclear componentsAbstract
This study investigated the interactions between nuclear components and the Dengue virus (DENV) core protein, focusing on their significance in viral replication. The nuclear proteins nucleolin, nucleophosmin, histone H2B, and RNA helicase DDX5 were identified as key players in enhancing DENV replication. Nucleolin, primarily involved in ribosomal RNA synthesis and processing, interacted with the DENV core protein to enhance viral RNA synthesis. Nucleophosmin, a critical factor in ribosome biogenesis and cellular stress response, facilitated viral particle assembly through its interaction with the core protein. Histone H2B, which plays a role in chromatin structure and gene regulation, modulated viral gene expression, optimizing the cellular environment for viral replication. RNA helicase DDX5, essential for RNA processing and modification, was found to promote the translation of viral RNA, further supporting the replication process. These interactions underscored the crucial role of nuclear components in the DENV lifecycle and provided insights into potential antiviral targets.
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